Searching with the sequence of hisA reveals significant homologies to hisF, aroG, fba II, trpC_2, trpA, guaB, pyc, pyrD and other protein families. The PSI-Blast results file is here
KHG/KDPG aldolase (eda) is a Schiff base dependent aldolase it is significantly similar to inosine monophosphate 5'-dehydrogenase (guaB). The PSI-Blast results file is here. Another family of Schiff base dependent enzymes is linked to trpA by KDG aldolase. PSI-Blast file here.
Lactate dehydrogenase (lctD) provides a bridge between dihydroorotate dehydrogenase (pyrD) and pyruvate kinase (pyk). PSI-Blast results here. Pyruvate kinase (pyk), phosphoenolpyruvate synthase (ppsA), and malate synthase (aceB) are linked by citrate lyase (citE). PSI-Blast results file here.
Muconate lactonizing enzyme (MLE) provides a bridge between NIR3 and enolase (eno). PSI-Blast results here. NIR3 found to be significantly similar to dihydroorotate dehydrogenase (pyrD). PSI-Blast results here here. These results also show a significant link to dihyropteroate synthase (folP).
The alpha subunit of tryptophan synthase (trpA) is significantly similar to triose phosphate isomerase (tpi). PSI-Blast results here. trpA is also detected by searches initiated with ribulose 3-phosphate epimerase (rpe), as is thiamin phosphate synthase (thiE). PSI-Blast results here.
Indole 3-Glycerol P-synthase (trpC_2) provides a significant link to
quinolate phosphoribosyltransferase (nadC). PSI-Blast results
here. nadC can be used in turn
to link to methylmalonyl-coA mutase (sbm). PSI-Blast results
here.
Results organised by Figure 1 keys:
hisA : HIS4_METVO
lctD : PDB:1ltd
citE : CILB_ECOLI
pyrD : PYRD_BACSU
MLE : PDB:1mucA
KDG ald: NR:2879782
eda : ALKH_ECOLI
nadC : NADC_METTH
rpe : RPE_SPIOL
trpA : TRPA_METTM
trpC : TRPC_SULSO