@article{Mende.2013.23892899, author = {Mende, DR and Sunagawa, S and Zeller, G and Bork, P}, title = {{Accurate and universal delineation of prokaryotic species}}, journal = {Nature methods}, pages = {}, year = {2013}, url = {http://www.ncbi.nlm.nih.gov/pubmed/23892899}, abstract = {{The exponentially increasing number of sequenced genomes necessitates fast, accurate, universally applicable and automated approaches for the delineation of prokaryotic species. We developed specI (species identification tool; http://www.bork.embl.de/software/specI/), a method to group organisms into species clusters based on 40 universal, single-copy phylogenetic marker genes. Applied to 3,496 prokaryotic genomes, specI identified 1,753 species clusters. Of 314 discrepancies with a widely used taxonomic classification, >62% were resolved by literature support.}} }