- In-frame stop codons: * or _ Examples: NGDLP*PQLKN or NGDLP_PQLKN - Frame shifts: by number Examples: Gene1 HCDGE Gene2 HC1GE only 1 nuc residue. Gene1 HCDGE Gene2 HC2GE only 2 nuc residues. Gene1 ND-TY Gene2 ND1TY 1 nuc residue inserted. Gene1 ND-TY Gene2 ND2TY 2 nuc residues inserted. Gene1 EREQK Gene2 EK4QK 1 nuc residue inserted.