long variant cdna alternative splice generate alternative splice alternatively splice transcripts splice variants alternative splice human gene alternative splice alternatively splice isoforms produce alternative splice alternatively splice alternative splice gene alternatively splice form alternative polyadenylation splice variant differential splice alternative splice variants polymerase chain reaction alternatively splice transcript alternatively splice exon alternative splice single splice variant human novel splice variant alternatively splice variant alternatively splice human alternative splice exon encode alternatively splice undergo alternative splice isoforms generate alternative derive alternative splice alternative splice generate identify alternatively splice alternate splice isoforms alternative splice splice gene splice acceptor site single gene splice transcripts splice single gene splice isoforms evidence alternative splice and alternatively splice alternative splice event alternatively splice encode transcripts @card@ @card@ single gene alternative splice human gene extensive alternative splice @card@ @card@ amino by alternative splice amino acid sequence alternative splice primary alternatively splice variants transcripts generate alternative regulate alternative splice alternative splice produce alternative splice mechanism alternatively splice isoform splice variant lack sequence alternative splice reverse chain reaction primary transcript human gene encode fibroblast growth factor alternative splice occur alternative splice involve alternative splice form alternative splice alternative 5 untranslated region splice variant gene novel alternatively splice @card@ alternatively splice alternative splice encode alternative splice @card@ splice primary transcript splice exon @card@ least alternatively splice full length transcript 3 untranslated region transcript alternative splice splice mechanism splice donor site several alternatively splice new splice variant expression alternative splice express alternatively splice encode @card@ amino and alternative splice alternative splice site alternatively splice region transcripts alternative splice subject alternative splice gene alternatively splice form alternative splice alternative splice transcript alternative splice acceptor alternative processing alternative exon usage originate alternative splice organization alternative splice northern blot reveal multiple alternatively splice gene expression endothelial growth factor @card@ amino and a single gene and @card@ amino alternative splice isoforms alternative splice identify alternative splice donor alternative splice common alternative splice 5 acidic fibroblast growth 5 splice site vascular endothelial growth structure alternative splice stop codon splice transcript encode splice form gene splice donor acceptor skeletal muscle several tissues represent alternatively splice generate by alternative generate alternative polyadenylation extracellular domain different splice variants different alternative splice contain open reading @card@ @card@ and a splice variant alternative splice transcripts alternative splice product alternative splice intron alternative splice domain alternatively splice gene alternatively splice @card@ alternative 5 splice acceptor site exon transcription start site splice site selection splice isoform splice form splice 5 end sequence alternatively splice reading frame probably alternative splice isoforms derive alternative isoforms @card@ @card@ human splice variant generate differential splice gene encode human full length human exon skip encode a protein code region alternative translation initiation alternative splice expression alternative splice different alternative splice code alternative splice affect alternative polyadenylation signal alternatively splice 5 truncated splice variant splice site exon splice gene encode splice form human splice exon splice code region specific alternative splice reveal alternative splice receptor splice variant possibly alternative splice organization human gene novel alternative splice genomic organization alternative exon 3 and deduce amino acid characterization alternatively splice alternative splice variant alternative splice region alternatively splice product alternatively splice express splice transcript splice 5 untranslated provide evidence alternative proteins alternative splice polyadenylation alternative splice novel splice variants multiple splice variants multiple alternative splice lack @card@ amino identify alternative splice generate multiple transcripts generate alternative exon gene differential splice exon alternatively splice existence alternative splice encode different isoforms domain alternative splice different protein isoforms different polyadenylation different isoforms differential splice generate @card@ amino contain carboxyl terminus a truncated protein appear alternatively splice an alternatively splice alternative usage polyadenylation alternative transcripts alternative splice regulate alternative splice pattern alternative splice differential alternative 3 end variants alternative splice variant lack exon transcripts differentially express transcript lack exon splice variants designate splice several tissues splice primary transcripts splice observe splice isoforms human splice exon skip splice event involve site selection short splice variant several splice variants role alternative splice rise alternatively splice regulation alternative splice protein generate alternative protein alternative splice product alternative splice produce single gene produce alternative polyadenylation pattern alternative splice isolate alternative splice isoform generate alternative involve alternative splice identify splice variant human skeletal muscle generate single gene generate alternate splice express splice variants expression alternatively splice express human tissues existence alternatively splice encode proteins @card@ donor splice site differential splice gene different human tissues derive alternate splice characterization alternative splice chain reaction amplification beta 1 syntrophin basis alternative splice and alternative 3 an alternative splice alternative splice third alternative splice production alternative splice contribute alternative splice 3 alternative polyadenylation alternative alternatively splice sequence alternatively splice observe alternatively splice generate alternative exon splice alternative exon additional splice variants 5 end 3 splice site 3 end variant lack @card@ upstream exon lead undergo extensive alternative two alternatively splice transcript single gene transcriptional start site third alternatively splice splice variants differentially splice variants different splice variants @card@ splice variant encode splice transcripts express splice transcripts encode splice site variants splice product splice precursor splice play role splice gene transcripts splice generate least splice generate large splice gene expression splice exon encode splice domain splice common precursor splice alternative splice splice acceptor skip alternative splice site exon single gene differential represent alternative splice region alternative splice presence alternatively splice possibility alternative splice originate single gene number proteins a northern reveal transcript mutually exclusive alternative mechanism alternative splice likely alternative splice include alternatively splice inactive alternatively splice gene reveal alternative generate protein isoforms generate large number gene encode alternatively expression splice variants expression human gene express alternative splice exon usage exon skip alternative exclusive alternative splice enzymatically inactive alternatively encode splice variants different produce alternative differential splice primary differential splice alternative differentially splice different alternatively splice differ alternative splice derive transcript single derive single gene demonstrate alternative splice complex alternative splice common precursor clone splice variants clone alternatively splice cell line encode cell adhesion molecule catalytic domain @card@ produce alternative @card@ @card@ ] @card@ amino short @card@ amino @card@ alternative splice precursor alternative splice polyadenylation alternative splice play alternative splice genomic alternative promoter utilization alternative promoter usage alternative messenger splice alternate splice gene 5 end exon 5 code region usage polyadenylation unusual splice event unknown function undergo complex alternative ubiquitously express @card@ two polyadenylation two alternative polyadenylation truncated protein transcripts differ 5 transcripts alternative polyadenylation transcription initiation third splice form splice variants tissue splice variants observe splice variants new splice variants lack splice variants identify splice variants generate splice variants express splice variants contain splice variant identify splice variant express splice intron 3 splice generate two splice generate multiple splice generate isoforms splice generate different splice extracellular domain splice event exon splice event splice encode soluble splice encode proteins splice alternative polyadenylation splice alternative initiation splice 3 end small transcripts generate pre mrna splice splice site mutation splice donor site splice acceptor site splice site intron 5 splice site splice site and @card@ donor splice site mrna splice 3 splice site amino acid substitution a missense mutation missense mutation exon mutation exon @card@ gt rule gene encode a splice site exon human and mouse deletion exon @card@ reverse chain reaction mutation codon @card@ reading frame @card@ human gene encode splice factor premature stop codon novel missense mutation exon intron splice alzheimer disease splice donor acceptor breast cancer 3 untranslated region strand conformation polymorphism acceptor site intron nonsense splice site conform gt rule entire code region autosomal dominant disorder acceptor splice site novel human gene missense mutation g a transition cryptic splice site site mutation contain open reading skip exon @card@ one splice site mutation a splice missense mutation a low density lipoprotein heart skeletal muscle consist @card@ amino code region gene @card@ patients autosomal recessive disorder substitution codon @card@ splice variant splice junction single base pair prostate cancer missense mutation gene fragment length polymorphism cancer cell a nonsense mutation tumour suppressor gene splice site @card@ splice mutation intron nucleotide position @card@ mental retardation @card@ unrelated patients @card@ patients @card@ @card@ missense nonsense @card@ genomic dna @card@ @card@ patients follow gt rule exon skip delta chain variant breast cancer cells base pair deletion bacterial artificial chromosome autosomal recessive disease a premature stop