Tutorials

Tutorial: Generate taxonomic profiles using mOTUs

This tutorial explains how to perform taxonomic profiling on your metagenomics samples.

Tutorial: Generate taxonomic profiles using mOTUs with MOCAT

This tutorial explains how to perform the same profiling from within MOCAT.

Stand-alone vs. MOCAT versions

Both methods perform the same computation. We recommend the stand-alone tool for casual use (with a small number of samples) and MOCAT for intensive use or when you need to change the intermediate steps for your data.

Advantages of the stand-alone version
  1. Download a single perl file (around 40 MB) which will automatically expand all dependencies.
  2. Run a single command to execute the whole pipeline.
  3. Only dependency is a working perl installation and basic utilities (standard with Linux or MAC OS).
Advantages of the MOCAT version
  1. Flexible option setting.
  2. Possibility of tweaking all intermediate steps.
  3. Can make use of computing clusters to distribute your work across many processors.
  4. All the functionality of MOCAT is available.