Computational Pipeline

To assess the differences in abundances of entire complex structures between male/female mice, and mice exposed to high-fat and chow diet, the median abundances of each complex was calculated in each individual/sample (protein subunits were required to be quantified in at least 50% of samples). For each complex it was then assessed via a t-test whether median complex abundances in male mice were significantly different from the ones in female mice; the effect size was monitored as the Cohen distance [Sullivan et al., 2012]. The applied t-test is two-sided as no directionality is implied. P-values were further adjusted using the Benjamini-Hochberg procedure (significance α = 0.05), and complex structures were considered significantly different in case of q-value < 0.01.